Difference between revisions of "Part:BBa K3335000"

Revision as of 12:14, 22 October 2020

We use KIBRA to control exosome secretion

KIBRA as an adaptor-like protein that stabilizes Rab27a, which in turn controls exosome secretion. Rab27a is stabilized by interacting with KIBRA, which prevents ubiquitination and degradation via the ubiquitin-proteasome pathway. KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a[1].

In our experiment, we used KIBRA to promote cell release of exosomes containing siRNA, thus increasing the efficiency of cell production and release of exosomes and improving the killing of tumor cells.

[1]Lin Song et al,.KIBRA controls exosome secretion via inhibiting the proteasomal degradation of Rab27a.Nature Communication,2019.

Usage and Biology

Contribution: NJU-China 2020

This year we want to use liver cells as a concept to treat lung cancer by overexpressing exosomes containing siRNA.

In our interview with the professor, we learned that exosome production is likely to be inefficient, so we found the KIBRA protein.KIBRA as an adaptor - like protein that stabilizes Rab27a, which in turn controls exosome secretion.

Figure1.KIBRA mRNA overexpression in HEK293T

KIBRA protein overexpressed in HEK293T

Western Blotting showed that KIBRA was overexpressed in HEK293T cells. We will further use it to verify the efficiency of promoting exosome release.

Sequence and Features

Functional Parameters