Difference between revisions of "Part:BBa K3506021"
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<partinfo>BBa_K3506021 parameters</partinfo>
 
<partinfo>BBa_K3506021 parameters</partinfo>
 
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<b><font size="3">References</font></b>
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[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016). https://doi.org/10.1038/srep31145
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[2]Li Q, Wang W, Zhao N, Li P, Xin Y, Hu W. Identification and validation of a Schistosoma japonicum U6 promoter. Parasit Vectors. 2017;10(1):281. Published 2017 Jun 5. doi:10.1186/s13071-017-2207-4

Revision as of 11:15, 22 October 2020


U6 promoter

U6 promoter is used to drive the expression of homing guide RNA（hgRNA） in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a clear transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3′ end of 2 -4 oligomeric U. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Wang, Y., Wei, D., Zhu, X. et al. A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans. Sci Rep 6, 31145 (2016). https://doi.org/10.1038/srep31145 [2]Li Q, Wang W, Zhao N, Li P, Xin Y, Hu W. Identification and validation of a Schistosoma japonicum U6 promoter. Parasit Vectors. 2017;10(1):281. Published 2017 Jun 5. doi:10.1186/s13071-017-2207-4