Difference between revisions of "Part:BBa K3506021"

 
Line 3: Line 3:
 
<partinfo>BBa_K3506021 short</partinfo>
 
<partinfo>BBa_K3506021 short</partinfo>
  
−
U6 promoter is a strong driver of gRNA production, and was used to drive the expression of sgRNA in lineage tracing for eukaryotic expression systems. We used it to initiate the transcription of hgRNA(sgRNA).The U6 promoter has a clear transcription start point G. All promoter elements are located upstream of the transcription start point. The 5&#8242;-TTTTT-3&#8242; sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3&#8242; end of 2 -4 oligomeric U.
+
U6 promoter is used to drive the expression of homing guide RNA（hgRNA） in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a clear transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3′ end of 2 -4 oligomeric U.<!-- Add more about the biology of this part here
−
 
+
−
<!-- Add more about the biology of this part here
+
 
===Usage and Biology===
 
===Usage and Biology===
 +
RNA polymerase III promoters uniquely transcribes small non-coding RNAs, including 5S rRNA , tRNAs, and other essential RNAs such as the U6 snRNA[1].U6 promoter is RNA polymerase III promoters and is a common part for eukaryotic expression systems. We use it to initiate the transcription of hgRNA used in CRISPR/Cas9 sysytem.You also can use the Pol Ⅲ promoter U6 to transcribe short hairpin RNA (shRNA,). The shRNA is cut by Dicer to become siRNA, which can interfere with the expression of target genes through the RNAi system.
  
 
<!-- -->
 
<!-- -->

Revision as of 11:05, 22 October 2020


U6 promoter

U6 promoter is used to drive the expression of homing guide RNA（hgRNA） in lineage tracing for eukaryotic systems. We used it to initiate the transcription of hgRNA(sgRNA). The U6 promoter has a clear transcription start point G. The 5′-TTTTT-3′ sequence located downstream of the promoter is used as a transcription termination signal, and the transcription product will be added at the 3′ end of 2 -4 oligomeric U. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]