Difference between revisions of "Part:BBa K3402057"

Revision as of 10:36, 22 October 2020

Double-gene editing cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), RS and HH(BBa_K3402029), sgPXA1(BBa_K3402039), HDV(BBa_K3402028), RS and HH(BBa_K3402029), sgGFP(BBa_K3402024), HDV(BBa_K3402028), Tsyn7(BBa_K3402001), upGFP(BBa_K3402025), doGFP(BBa_K3402026).

File:.png

Usage and Biology

We realize the knock-out of PXA1 and yeGFP genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2171
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 2807

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 This device is composed of 50bp-up<i>PXA1</i>(BBa_K3402037), <i>hph</i>(BBa_K3402012), 50bp-do<i>PXA1</i>(BBa_K3402038), P<i>tef1</i>(BBa_K3402007), <i>RS</i> and <i>HH</i>(BBa_K3402029), sg<i>PXA1</i>(BBa_K3402039), <i>HDV</i>(BBa_K3402028), <i>RS</i> and <i>HH</i>(BBa_K3402029), sg<i>GFP</i>(BBa_K3402024), <i>HDV</i>(BBa_K3402028), T<i>syn7</i>(BBa_K3402001), up<i>GFP</i>(BBa_K3402025), do<i>GFP</i>(BBa_K3402026).
-<!-- Add more about the biology of this part here
+[[Image:.png|700px|center|]]
 ===Usage and Biology===
+We realize the knock-out of <i>PXA1</i> and <i>yeGFP</i> genes. After observing the strains that growed on the plate coated with hygromycin can also lose the function of expressing green fluorescence, we got the conclusion that the success of double gene editing was proved.
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