Difference between revisions of "Part:BBa K3402056"
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If modified strains can grow on the plate coated with hygromycin, it is successful to knock <i>PXA1</i> with Cas9 system. By comparison, the editing efficiency of the CRISPR/Cas9 system can be clearly observed.
 
If modified strains can grow on the plate coated with hygromycin, it is successful to knock <i>PXA1</i> with Cas9 system. By comparison, the editing efficiency of the CRISPR/Cas9 system can be clearly observed.
  
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[[Image:Single-gene editing cassette.png|700px|thumb|center]]
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[[Image:Single-gene editing cassette.png|500px|thumb|center]]
  
  

Revision as of 10:28, 22 October 2020


Single-gene editing cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), hph(BBa_K3402012), 50bp-doPXA1(BBa_K3402038), Ptef1(BBa_K3402007), RS and HH(BBa_K3402029), sgPXA1(BBa_K3402039), HDV(BBa_K3402028), Tsyn7(BBa_K3402001).

File:.png

Usage and Biology

We achieve the knockout of PXA1 gene and insert the hygromycin resistant gene as a selection marker. Two parts of PXA1 are homologous arms at the ends.
If modified strains can grow on the plate coated with hygromycin, it is successful to knock PXA1 with Cas9 system. By comparison, the editing efficiency of the CRISPR/Cas9 system can be clearly observed.

Single-gene editing cassette.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]