Difference between revisions of "Part:BBa K3408004"
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https://2020.igem.org/wiki/images/5/5e/T--NAU-CHINA--basic-parts4.png | https://2020.igem.org/wiki/images/5/5e/T--NAU-CHINA--basic-parts4.png | ||
| − | Fig.1. Codon quality distribution | + | Fig.1. Codon quality distribution &nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp; Fig.2. Optimized codon quality distribution |
The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987). | The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987). | ||
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https://2020.igem.org/wiki/images/7/77/T--NAU-CHINA--basic-parts6.png | https://2020.igem.org/wiki/images/7/77/T--NAU-CHINA--basic-parts6.png | ||
| − | Fig.3. Codon quality plot | + | Fig.3. Codon quality plot &nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp; Fig.4. Optimized codon quality plot |
The plots show the quality of the used codon at the indicated codon position. | The plots show the quality of the used codon at the indicated codon position. | ||
Revision as of 08:59, 22 October 2020
Optimized CⅠ
It is a protein which could suppress PC1 so that our switch RNA couldn't be transcribed without oxygen.
We designed it under the control of Pnar.
We have optimized the C1 protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.
Fig.1. Codon quality distribution &nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp; Fig.2. Optimized codon quality distribution
The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).
Fig.3. Codon quality plot &nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp;&nsp; Fig.4. Optimized codon quality plot
The plots show the quality of the used codon at the indicated codon position.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]