Difference between revisions of "Part:BBa K3447007"
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<partinfo>BBa_K3447007 short</partinfo> | <partinfo>BBa_K3447007 short</partinfo> | ||
− | + | P<sub>BAD</sub> promoter is an inducible promoter, P<sub>BAD</sub> contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only <i>araC</i> and P<sub>BAD</sub>.<br> | |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | In the arabinose operon, it is regulated by the AraC operon and activates the expression of downstream arabinose metabolism genes in the presence of arabinose inducers.<br> | ||
+ | The L-Arabinose operon is naturally present in <i>E. coli</i>.The regulatory protein AraC ACTS as a dimer. The operon contains two cis-type control elements: The P<sub>C</sub> promoter for the synthesis of AraC and the P<sub>BAD</sub> for the synthesis of the enzyme needed for the catabolism of L-Arabinose. P<sub>BAD</sub> contains three half sites, each of which binds to a subunit of ARAC-O2, I1, and I2. The O1 site is composed of O1L and O1R half sites that bind two subunits. Half of O2 is in the araC coding region. | ||
+ | We connected the target genes downstream of P<sub>BAD</sub> to constitute the effect part of arabinose sensing system, and constructed the whole arabinose sensing system on the same plasmid with P<sub>C</sub> and araC. | ||
+ | Arabinose induces endotoxin expression: <partinfo>BBa_K3447000</partinfo> | ||
+ | Arabinose induces green fluorescent protein expression: (链接a01+c09) | ||
+ | |||
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Revision as of 08:47, 22 October 2020
xynD, which produces xylanase
PBAD promoter is an inducible promoter, PBAD contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only araC and PBAD.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 216
Illegal PstI site found at 226 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 216
Illegal PstI site found at 226 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 216
Illegal PstI site found at 226 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 216
Illegal PstI site found at 226 - 1000COMPATIBLE WITH RFC[1000]