Difference between revisions of "Part:BBa K3447006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We initiated the expression of downstream sfGFP <BBa_K2541400> and repressor protein CI BBa_C0051 by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.<br> | + | We initiated the expression of downstream sfGFP <partinfo>BBa_K2541400</partinfo> and repressor protein CI <partinfo>BBa_C0051</partinfo> by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.<br> |
* Connect sfGFP gene downstream of fixK2 promoter: 链接<br> | * Connect sfGFP gene downstream of fixK2 promoter: 链接<br> | ||
* Connect repressor proteins CI downstream of fixK2 promoter: 链接<br><br> | * Connect repressor proteins CI downstream of fixK2 promoter: 链接<br><br> | ||
Revision as of 06:05, 22 October 2020
xynD, which produces xylanase
FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
Usage and Biology
We initiated the expression of downstream sfGFP BBa_K2541400 and repressor protein CI BBa_C0051 by connecting the chromogenic group (链接) of the blue light photosensitive system upstream of fixK2 promoters.
- Connect sfGFP gene downstream of fixK2 promoter: 链接
- Connect repressor proteins CI downstream of fixK2 promoter: 链接
Design
Design Notes
We added some synonymous mutations to avoid part rules.
Source
We found this sequence data in the previous iGEM teams (UCAS-China 2018 and Uppsala-Sweden 2011) and in GenBank.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 8
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 83
- 1000COMPATIBLE WITH RFC[1000]