Difference between revisions of "Part:BBa K3408003"
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It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P<sub>CI</sub> when oxygen exists in wormcast.
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It is the part of toehold switch which functions as the controller of suicide switch in our project. It could form a hairpin to block circuit and prevent downstream gene from being translated.
  
 
We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the switch RNA to mazF, following the P<sub>CI</sub> downstream
 
We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the switch RNA to mazF, following the P<sub>CI</sub> downstream

Revision as of 04:50, 22 October 2020

Switch RNA(of toehold)


It is the part of toehold switch which functions as the controller of suicide switch in our project. It could form a hairpin to block circuit and prevent downstream gene from being translated.

We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the switch RNA to mazF, following the PCI downstream

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]