Difference between revisions of "Part:BBa K3408003"
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It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P<sub>CI</sub> when oxygen exists in wormcast. | It is the part of toehold switch which functions as the controller of suicide switch. It is activated by P<sub>CI</sub> when oxygen exists in wormcast. | ||
| − | We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the | + | We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the switch RNA to mazF, following the P<sub>CI</sub> downstream |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 04:37, 22 October 2020
Switch RNA(of toehold)
It is the part of toehold switch which functions as the controller of suicide switch. It is activated by PCI when oxygen exists in wormcast.
We hope our engineered bacteria not express MazF protein accidentally without having functioned yet, while we also couldn’t avoid the environment with oxygen when we cultivate them in the lab. So we added the switch RNA to mazF, following the PCI downstream
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 8
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]