Difference between revisions of "Part:BBa K3408002"
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https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
https://2020.igem.org/wiki/images/4/48/T--NAU-CHINA--basic-parts2.png
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
 
<span style="text-align:center; display:inline-block;">Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., <i>Biotechnology Progress</i>. 2010)</span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3408002 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3408002 parameters</partinfo>
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Revision as of 04:33, 22 October 2020

Trigger RNA(of toehold)


We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.

We modifed our Trigger RNA with some bps in the 5’ terminal to increase its stability.

T--NAU-CHINA--basic-parts2.png Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]