Difference between revisions of "Part:BBa K3408001"
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According to the paper, we could see its <b>optimum reaction temperature is 45℃</b>, Km is 0.213mmol/L and Vmax is 5.55U/mg.
 
According to the paper, we could see its <b>optimum reaction temperature is 45℃</b>, Km is 0.213mmol/L and Vmax is 5.55U/mg.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3408003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3408003 parameters</partinfo>
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Revision as of 04:31, 22 October 2020

Phy(ycD)(Phytase)


Because of the limitation of pH, we have to choose the phosphate-solubilizing enzyme which could work efficiently between the pH from pH6.0 to pH7.0. And we have found the phy(ycD) whose optimum reaction pH is pH6.5. Thus, we added it into the anaerobic regulation circuit, following the Pnar.

T--NAU-CHINA--RA.jpg

Fig.2. Phy (ycD) activity changes with temperature(Wang Xi, et al., Applied and Environmental Biology. 2014)


According to the paper, we could see its optimum reaction temperature is 45℃, Km is 0.213mmol/L and Vmax is 5.55U/mg.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]