Difference between revisions of "Part:BBa K3408002"
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We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide. | We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide. | ||
Revision as of 04:29, 22 October 2020
Trigger RNA(of toehold)
We would use the Trigger NRA to release mazF when switch-mazF is expressed in wormcast. So our engineered bacterium could commit suicide.
We modifed our Trigger RNA with some bps in the 5’ terminal to increase its stability.
Fig.3. Effect of different Synthetic 5′Secondary Structures to half-life of mRNA(Carrier T A, et al., Biotechnology Progress. 2010)