Difference between revisions of "Part:BBa K3510000"

 
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<partinfo>BBa_K3510000 short</partinfo>
 
<partinfo>BBa_K3510000 short</partinfo>
  
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Fluorescence-Activating and Absorption-Shifting Tag Protein 2(FAST2) reporter gene as developed by the Twinkle Factory. The FAST2 Tag allows for efficient, switchable fluorescent labeling of any protein of interest in aerobic and anaerobic environments upon the complementation with a fluorogen.
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This part encodes for the Fluorescence-Activating and Absorption-Shifting Tag (FAST) protein, a 14 kDAsmall protein, that  assembles with various fluorgenic ligands (Fluorogens) [1]. Only upon assembly a fluorescent signal is emitted and allows for detection and imaging with high contrast [1].  
  
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The original sequence was obtained from the Twinkle Factory and edited in one base pair to eliminate a PstI binding site, so it now follows iGEM’s assembly rules. In our project it was used as a fluorescent tag of the following DNA fragment, either the phytochelatin or chromoprotein.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3510000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3510000 SequenceAndFeatures</partinfo>
  
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==References==
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#The Twinkle Factory. The science behind FAST and SplitFAST [cited 2020 Oct 19]. Available from: URL: https://www.the-twinkle-factory.com/the-science-behind-fast-and-splitfast.
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===Usage and Biology===
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Fluorescence-Activating and Absorption-Shifting Tag Protein 2(FAST2) reporter gene as developed by the Twinkle Factory. The FAST2 Tag allows for efficient, switchable fluorescent labeling of any protein of interest in aerobic and anaerobic environments upon the complementation with a fluorogen.
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Revision as of 08:03, 21 October 2020

FAST2 reporter gene

This part encodes for the Fluorescence-Activating and Absorption-Shifting Tag (FAST) protein, a 14 kDAsmall protein, that assembles with various fluorgenic ligands (Fluorogens) [1]. Only upon assembly a fluorescent signal is emitted and allows for detection and imaging with high contrast [1].

The original sequence was obtained from the Twinkle Factory and edited in one base pair to eliminate a PstI binding site, so it now follows iGEM’s assembly rules. In our project it was used as a fluorescent tag of the following DNA fragment, either the phytochelatin or chromoprotein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. The Twinkle Factory. The science behind FAST and SplitFAST [cited 2020 Oct 19]. Available from: URL: https://www.the-twinkle-factory.com/the-science-behind-fast-and-splitfast.