Difference between revisions of "Part:BBa K143036:Design"

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__NOTOC__
 
__NOTOC__
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===<big>Xylose operon regulatory protein</big>===
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<partinfo>BBa_K143036 short</partinfo>
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<br>
  
 
<partinfo>BBa_K143036 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143036 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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The XylR protein was identified in the genome using its Genbank entry<cite>#2</cite> and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase
 
  
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The wild-type XylR protein was identified using the complete genome sequene of B. subtilis <cite>#1</cite> and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.
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====Template====
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''B. subtilis'' Chromosome
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*We thank Dr. Angelika Grundling, Centre for Molecular Microbiology and Infection, Imperial College for providing the chromosome preparation for our PCR
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====Forward Primer====
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5'- GCT CTA GAT GAC TGG ATT AAA TAA ATC AAC - 3'
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====Reverse Primer====
  
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5'- GGA CTA GTA TTA TTA CAT TGT AAT CAT GTC CAG -3'
  
 
===Source===
 
===Source===
  
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The XylR protein was PCR cloned form the ''B. subtilis''  genome using Pfu DNA polymerase
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The XylR protein was PCR cloned from the ''B. subtilis''  genome using Pfu DNA polymerase
  
 
===References===
 
===References===
 
<biblio>
 
<biblio>
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#1 pmid=2544559
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#1 pmid=9384377
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#2 http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, Gene ID:939531
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</biblio>
 
</biblio>

Latest revision as of 00:23, 29 October 2008

Xylose operon regulatory protein



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The wild-type XylR protein was identified using the complete genome sequene of B. subtilis #1 and PCR primers designed from this sequence. Biobrick prefix and suffix standards were added to the XylR sequence and the gene cloned by PCR with Pfu DNA polymerase.

Template

B. subtilis Chromosome

  • We thank Dr. Angelika Grundling, Centre for Molecular Microbiology and Infection, Imperial College for providing the chromosome preparation for our PCR

Forward Primer

5'- GCT CTA GAT GAC TGG ATT AAA TAA ATC AAC - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA CAT TGT AAT CAT GTC CAG -3'

Source

The XylR protein was PCR cloned from the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=9384377

</biblio>