Difference between revisions of "Part:BBa K143033:Design"

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===Design Notes===
 
===Design Notes===
 
LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
 
LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
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====Template====
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''B. subtilis'' integration plasmid pDR111<cite>#1</cite>
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 +
====Forward Primer====
 +
 +
5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3'
 +
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====Reverse Primer====
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5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3'
  
 
===Source===
 
===Source===
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<biblio>
 
<biblio>
−
#1 pmid=16166525
+
#1 pmid=12562810
 
</biblio>
 
</biblio>

Latest revision as of 00:22, 29 October 2008

LacI (Lva-, N-terminal deletion) regulatory protein



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.

Template

B. subtilis integration plasmid pDR111#1

Forward Primer

5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3'

Source

The LacI gene was cloned from the B. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR.

References

<biblio>

  1. 1 pmid=12562810

</biblio>