Difference between revisions of "Part:BBa K143033:Design"

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__NOTOC__
 
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<partinfo>BBa_K143033 short</partinfo>
 
<partinfo>BBa_K143033 short</partinfo>
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<br>
  
 
<partinfo>BBa_K143033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143033 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks a Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
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LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
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====Template====
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''B. subtilis'' integration plasmid pDR111<cite>#1</cite>
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====Forward Primer====
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5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3'
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====Reverse Primer====
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5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3'
  
 
===Source===
 
===Source===
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<biblio>
 
<biblio>
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#1 pmid=16166525
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#1 pmid=12562810
 
</biblio>
 
</biblio>

Latest revision as of 00:22, 29 October 2008

LacI (Lva-, N-terminal deletion) regulatory protein



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.

Template

B. subtilis integration plasmid pDR111#1

Forward Primer

5'- GCT CTA GAT GAA ACC AGT AAC GTT ATA CGA TG - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA CTG CCC GCT TTC CAG TC -3'

Source

The LacI gene was cloned from the B. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR.

References

<biblio>

  1. 1 pmid=12562810

</biblio>