Difference between revisions of "Part:BBa K143031:Design"
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<partinfo>BBa_K143031 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143031 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
+ | |||
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning | The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning | ||
+ | ====Template==== | ||
+ | |||
+ | ''B. subtilis'' integration plasmid pDR111<cite>#1</cite> | ||
+ | |||
+ | ====Forward Primer==== | ||
+ | |||
+ | 5'- GCT CTA GAT GAG GAG GAT ATA TTT GAA TAC - 3' | ||
+ | |||
+ | ====Reverse Primer==== | ||
+ | |||
+ | 5'- GGA CTA GTA TTA TTA TAA TTT TTT TAA TCT GTT ATT TAA ATA G -3' | ||
===Source=== | ===Source=== | ||
Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase | Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase | ||
+ | |||
+ | ===References=== | ||
+ | |||
+ | <biblio> | ||
+ | #1 pmid=12562810 | ||
+ | </biblio> |
Latest revision as of 00:22, 29 October 2008
Aad9 Spectinomycin Resistance Gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 488
Design Notes
The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
Template
B. subtilis integration plasmid pDR111#1
Forward Primer
5'- GCT CTA GAT GAG GAG GAT ATA TTT GAA TAC - 3'
Reverse Primer
5'- GGA CTA GTA TTA TTA TAA TTT TTT TAA TCT GTT ATT TAA ATA G -3'
Source
Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase
References
<biblio>
- 1 pmid=12562810
</biblio>