Difference between revisions of "Part:BBa K143031:Design"

m
m
 
(3 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K143031 short</partinfo>
 
<partinfo>BBa_K143031 short</partinfo>
 +
 +
<br>
  
 
<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143031 SequenceAndFeatures</partinfo>
−
 
  
 
===Design Notes===
 
===Design Notes===
 +
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
 
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
  
 +
====Template====
 +
 +
''B. subtilis'' integration plasmid pDR111<cite>#1</cite>
 +
 +
====Forward Primer====
 +
 +
5'- GCT CTA GAT GAG GAG GAT ATA TTT GAA TAC - 3'
 +
 +
====Reverse Primer====
  
 +
5'- GGA CTA GTA TTA TTA TAA TTT TTT TAA TCT GTT ATT TAA ATA G -3'
  
 
===Source===
 
===Source===
Line 17: Line 29:
  
 
<biblio>
 
<biblio>
−
#1 pmid=1659306
+
#1 pmid=12562810
−
#2 pmid=16997985
+
−
#3 pmid=15060042
+
−
#4 pmid=16714443
+
 
</biblio>
 
</biblio>

Latest revision as of 00:22, 29 October 2008

Aad9 Spectinomycin Resistance Gene



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488

Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning

Template

B. subtilis integration plasmid pDR111#1

Forward Primer

5'- GCT CTA GAT GAG GAG GAT ATA TTT GAA TAC - 3'

Reverse Primer

5'- GGA CTA GTA TTA TTA TAA TTT TTT TAA TCT GTT ATT TAA ATA G -3'

Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=12562810

</biblio>