Difference between revisions of "Part:BBa K143002:Design"

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<big>'''3' Integration sequence for the AmyE locus of ''B. subtilis'''''</big>
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__NOTOC__
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===<big>'''3' Integration sequence for the amyE locus of ''B. subtilis'''''</big>===
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<br>
  
 
<partinfo>BBa_K143002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143002 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
  
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The AmyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration  
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The amyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration  
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====Template====
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''B. subtilis'' integration plasmid pDR111<cite>#1</cite>
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====Forward Primer====
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5'- GCT CTA GAG ATC CGT TTA GGC TGG GC - 3'
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====Reverse Primer====
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5'- GGA CTA GTA TCA ATG GGG AAG AGA ACC G -3'
  
 
===Source===
 
===Source===
  
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The 5’ integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B. subtilis'', in particular in the studies of transcriptional control<cite>#1,#2,#3</cite>  
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The 5’ integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on ''B. subtilis'', in particular in the studies of transcriptional control<cite>#2,#3,#4</cite>  
  
 
===References===
 
===References===
  
 
<biblio>
 
<biblio>
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#1 pmid=14597697
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#1 pmid=12562810
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#2 pmid=15937167
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#2 pmid=14597697
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#3 pmid=12169614
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#3 pmid=15937167
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#4 pmid=12169614
 
</biblio>
 
</biblio>

Latest revision as of 00:19, 29 October 2008

3' Integration sequence for the amyE locus of B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

The amyE integration sequence was taken from the vector after comparison by BLAST to the B.subtilis chromosome to identify the homologous sequences. The sequence present in both the host chromosome and the plasmid at the 3' end of the gene is the 3' sequence required for integration

Template

B. subtilis integration plasmid pDR111#1

Forward Primer

5'- GCT CTA GAG ATC CGT TTA GGC TGG GC - 3'

Reverse Primer

5'- GGA CTA GTA TCA ATG GGG AAG AGA ACC G -3'

Source

The 5’ integration sequence was taken from the shuttle vector pDR111 which has been used in many studies on B. subtilis, in particular in the studies of transcriptional control#2,#3,#4

References

<biblio>

  1. 1 pmid=12562810
  2. 2 pmid=14597697
  3. 3 pmid=15937167
  4. 4 pmid=12169614

</biblio>