Difference between revisions of "Part:BBa K3381002:Design"
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<partinfo>BBa_K3381002 short</partinfo>
 
<partinfo>BBa_K3381002 short</partinfo>
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Methylosinus trichosporium O3B, sequence found through GenBank https://www.ncbi.nlm.nih.gov/protein/ATQ66671
 
Methylosinus trichosporium O3B, sequence found through GenBank https://www.ncbi.nlm.nih.gov/protein/ATQ66671
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===References===
 
===References===
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Lawton, T. J., Kenney, G. E., Hurley, J. D., & Rosenzweig, A. C. (2016). The CopC Family: Structural and Bioinformatic Insights into a Diverse Group of Periplasmic Copper Binding Proteins. Biochemistry, 55(15), 2278-2290. doi:10.1021/acs.biochem.6b00175.s003

Revision as of 01:26, 21 October 2020

Mst-CopC (copper-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.


Source

Methylosinus trichosporium O3B, sequence found through GenBank https://www.ncbi.nlm.nih.gov/protein/ATQ66671


References

Lawton, T. J., Kenney, G. E., Hurley, J. D., & Rosenzweig, A. C. (2016). The CopC Family: Structural and Bioinformatic Insights into a Diverse Group of Periplasmic Copper Binding Proteins. Biochemistry, 55(15), 2278-2290. doi:10.1021/acs.biochem.6b00175.s003