Difference between revisions of "Part:BBa K3381000:Design"

 
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3381000 short</partinfo>
 
<partinfo>BBa_K3381000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for *E. coli*, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.
+
The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.
−
 
+
  
  
 
===Source===
 
===Source===
−
 
 
Synechocystis sp. PCC6803, sequence found through GenBank: https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=291626&to=292708  
 
Synechocystis sp. PCC6803, sequence found through GenBank: https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=291626&to=292708  
 +
  
 
===References===
 
===References===
 +
Badarau, A., Firbank, S.J., Waldron, K.J., Yanagisawa, S., Robinson N.J., Banfield, M.J., & Dennison, C. (2008). FutA2 is a ferric binding protein from Synechocystis PCC 6803. Journal of Biological Chemistry, 283(18), 12520-7. https://doi.org/10.1074/jbc.M709907200
 +
 +
Katoh, H., Hagino, N., & Ogawa, T. (2001). Iron-Binding Activity of FutA1 Subunit of an ABC-type Iron Transporter in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Plant and Cell Physiology, 42(8). 823–827, https://doi.org/10.1093/pcp/pce106
 +
 +
Koropatkin, N. et al. (2007). The Structure of the Iron-binding Protein, FutA1, from Synechocystis 6803. Journal of Biological Chemistry, 282, 27468-27477. https://10.1074/jbc.M704136200

Latest revision as of 01:22, 21 October 2020

FutA1 (iron-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 414
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The periplasmic localization tags were determined by the SignalP-5.0 Server and removed. The sequences were then codon optimized for E. coli, removing all cut sites for RFc 25 and NdeI/BamHI (required for cloning into pET 11a). Lastly, the prefix and suffix for RFc 25 were appended.


Source

Synechocystis sp. PCC6803, sequence found through GenBank: https://www.ncbi.nlm.nih.gov/nuccore/BA000022.2?from=291626&to=292708


References

Badarau, A., Firbank, S.J., Waldron, K.J., Yanagisawa, S., Robinson N.J., Banfield, M.J., & Dennison, C. (2008). FutA2 is a ferric binding protein from Synechocystis PCC 6803. Journal of Biological Chemistry, 283(18), 12520-7. https://doi.org/10.1074/jbc.M709907200

Katoh, H., Hagino, N., & Ogawa, T. (2001). Iron-Binding Activity of FutA1 Subunit of an ABC-type Iron Transporter in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Plant and Cell Physiology, 42(8). 823–827, https://doi.org/10.1093/pcp/pce106

Koropatkin, N. et al. (2007). The Structure of the Iron-binding Protein, FutA1, from Synechocystis 6803. Journal of Biological Chemistry, 282, 27468-27477. https://10.1074/jbc.M704136200