Difference between revisions of "Part:BBa K3582000:Design"

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===References===
 
===References===
 +
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*[1] Abraham G, Colonno RJ (Aug 1984). "Many rhinovirus serotypes share the same cellular receptor". Journal of Virology. 51 (2): 340–45. doi:10.1128/JVI.51.2.340-345.1984. PMC 254443. PMID 6086949.
 +
*[2] Rothlein R, Dustin ML, Marlin SD, Springer TA (August 1986). "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1". Journal of Immunology. 137 (4): 1270–4. PMID 3525675.
 +
*[3]Yang L, Froio RM, Sciuto TE, Dvorak AM, Alon R, Luscinskas FW (July 2005). "ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium underflow". Blood. 106 (2): 584–92. doi:10.1182/blood-2004-12-4942. PMC 1635241. PMID 15811956
 +
*[4] Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
 +
*[5]https://www.uniprot.org/uniprot/P05362

Revision as of 16:07, 20 October 2020


ICAM-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • 1] Strep-tag was added at the end of the biobrick for ease of purification.
  • 2] The sequence was codon optimized with the E.coli K-12 strain.
  • 3] RFC10 criteria satisfied

Source

The protein sequence was taken from PDB and was reverse translated to give this biobrick

References

  • [1] Abraham G, Colonno RJ (Aug 1984). "Many rhinovirus serotypes share the same cellular receptor". Journal of Virology. 51 (2): 340–45. doi:10.1128/JVI.51.2.340-345.1984. PMC 254443. PMID 6086949.
  • [2] Rothlein R, Dustin ML, Marlin SD, Springer TA (August 1986). "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1". Journal of Immunology. 137 (4): 1270–4. PMID 3525675.
  • [3]Yang L, Froio RM, Sciuto TE, Dvorak AM, Alon R, Luscinskas FW (July 2005). "ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium underflow". Blood. 106 (2): 584–92. doi:10.1182/blood-2004-12-4942. PMC 1635241. PMID 15811956
  • [4] Staunton DE, Marlin SD, Stratowa C, Dustin ML, Springer TA. Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. Cell. 1988;52:925–933.
  • [5]https://www.uniprot.org/uniprot/P05362