Difference between revisions of "Part:BBa K3328008:Experience"
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===Applications of BBa_K3328013=== | ===Applications of BBa_K3328013=== | ||
| − | https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg | + | <img src="https://2020.igem.org/wiki/images/7/7e/T--OUC-China--result_fig2_th.jpg" style="width:80%" /> |
Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure. | Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure. | ||
Revision as of 15:51, 20 October 2020
Toehold switch is composed of cis-acting element RNA hairpins and trans-acting factor trigger RNA. The binding of a trigger RNA to the toehold sequence allows for a branch migration process, exposing AUG and RBS for translation initiation.
Applications of BBa_K3328013
<img src="
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Compared with the blank control (IPTG=0 M, aTc=0 mg/ml), the fluorescence of GFP was low when only the promoter before toehold sequence was turned on (IPTG=0.1 M, aTc=0 mg/ml). This indicates that the toehold has the advantage of low leakage. When the trigger was expressed (IPTG=0.1 M, aTc=0.25 mg/ml), it showed a high GFP fluorescence of up to 32.3-fold due to the destruction of toehold hairpin structure.
User Reviews
UNIQa71af78d7ea367ea-partinfo-00000000-QINU UNIQa71af78d7ea367ea-partinfo-00000001-QINU