Difference between revisions of "Part:BBa K3482004"
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Revision as of 15:18, 20 October 2020
IPTG-inducible miniColicin toxin
This part allows for IPTG inducible expression of the DNAase miniColicin in E. coli. The degradation of DNA , which leads to cell death, can therefore be induced by IPTG.
Biology
Colicin E2 is an endonuclease that cut on both single and double-stranded DNA. It has no specific cutting site. Colicin toxin are toxins that can be produced by E.coli and closely related bacteria.
Usage
We used that part to test if the expression of the toxin work in our E.coli nissle 1917 strain and if it was sufficient do provoc cell death
Characterization
File:BBa K3482004 kill-switch plate.File:File.jpg
Functional killswitch assay with IPTG and aTc gradients on agar plate
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 124
- 1000COMPATIBLE WITH RFC[1000]