Difference between revisions of "Part:BBa K3582000:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
1] Strep tag was added at the end of the biobrick for ease of purification
+
1] Strep tag was added at the end of the biobrick for ease of purification//
2] The sequence was codon optimised with the E.coli K-12 strain
+
2] The sequence was codon optimised with the E.coli K-12 strain//
 
3] RFC10 criteria satisfied
 
3] RFC10 criteria satisfied
 
 
  
 
===Source===
 
===Source===

Revision as of 14:34, 20 October 2020


ICAM-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1] Strep tag was added at the end of the biobrick for ease of purification// 2] The sequence was codon optimised with the E.coli K-12 strain// 3] RFC10 criteria satisfied

Source

The protein sequence was taken from PDB and was reverse translated to give this biobrick

References