Difference between revisions of "Part:BBa K2622004"
 
(2 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2622004 short</partinfo>
 
<partinfo>BBa_K2622004 short</partinfo>
  
−
Single chain antibody against vaginolysin
+
Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.
 +
 
 +
This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.
 +
 
 +
==References==
 +
 
 +
Pleckaityte, M., Mistiniene, E., Lasickiene, R., Zvirblis, G., & Zvirbliene, A. (2011). Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis. BMC biotechnology, 11, 100. https://doi.org/10.1186/1472-6750-11-100
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:29, 20 October 2020


scFv_antiVGY

Single chain antibody against vaginolysin. It has heavy chain connected to a heavy chain via gly/ser rich linker. This single chain binds to the vaginolysin and inhibts its lysation of cholesterol rich membranes. It could be used in erythrocyte lysis assay.

This biobrick has RFC10 prefix and suffix and dinucleotides - AT at the start of the part and GC at the end of the part that are used for protein fusion with RFC12 biobricks in order to avoid ORF changes.

References

Pleckaityte, M., Mistiniene, E., Lasickiene, R., Zvirblis, G., & Zvirbliene, A. (2011). Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis. BMC biotechnology, 11, 100. https://doi.org/10.1186/1472-6750-11-100

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41