Difference between revisions of "Part:BBa K103004"
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<partinfo>BBa_K103004 short</partinfo> | <partinfo>BBa_K103004 short</partinfo> | ||
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| − | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003)[http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum] | + | Artificially created strong interactor of B domain of Staphylococcal protein A ([[Part:BBa_K103003 | BBa_K103003]]). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]. |
*Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | *Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | ||
*We have used Z<sub>SPA-1</sub> protein fusions with [[Part:BBa_K103019 | BBa_K103019]] or [[Part:BBa_K103020 | BBa_K103020]] as a prey in our 'hunter and prey' selection system | *We have used Z<sub>SPA-1</sub> protein fusions with [[Part:BBa_K103019 | BBa_K103019]] or [[Part:BBa_K103020 | BBa_K103020]] as a prey in our 'hunter and prey' selection system | ||
*This part uses our NdeI/SacI/BamHI fusion cloning substandard | *This part uses our NdeI/SacI/BamHI fusion cloning substandard | ||
| − | + | |[[Image:Z_spa.jpg]] | |
| + | Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB] | ||
| + | |} | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 23:01, 28 October 2008
protein ZSPA-1
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Artificially created strong interactor of B domain of Staphylococcal protein A ( BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A [http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum].
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Source: [http://www.pdb.org/pdb/explore.do?structureId=2B88 PDB] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]