Difference between revisions of "Part:BBa K3610039:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Latest revision as of 10:59, 20 October 2020
EFR ectodomain / mCherry N-terminal for S. cerevisiae
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 355
Illegal NheI site found at 1285
Illegal NheI site found at 2203 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1871
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
HA tag added between linker and mCherry for Western Blotting
Source
BBa_K319005, BBa_K2809002, BBa_K3610007, BBa_K416001, BBa_K1150016, BBa_K3610020, BBa_K2753050
References
Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.
Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.