Difference between revisions of "Part:BBa K3487007"
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===Result=== | ===Result=== | ||
We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following. | We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following. | ||
| − | [[File:T--SZPT-CHINA--RS2.png| | + | [[File:T--SZPT-CHINA--RS2.png|thumb|700px|center|Relationship between Lysine Concentration and Fluorescence Intensity in Lysine Riboswitch]] |
The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally. | The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally. | ||
Revision as of 08:56, 20 October 2020
Lysine riboswitch
Description
Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch.RS is a Lysine riboswitch that Regulated by lysine. The following is the control principle diagram
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 12
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 284
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 256
2020 SZPT-CHINA
Result
We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following.
The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally.
References
Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.