Difference between revisions of "Part:BBa K3487007"
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===Description===
 
===Description===
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Usually the suicide switch that prevents organisms from escaping will be set to auxotrophic type, which requires gene resection technology, and whether it is accurate is a big problem that needs to be faced. The riboswitch does not have these problems. The gene sequence is directly introduced into the required vector, and the switch directly forms a multiple stem-loop structure on the mRNA to stop translation.
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Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch.RS is a Lysine riboswitch that Regulated by lysine.
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[[File:T--SZPT-CHINA--RS.png|900px|center|]]
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The following is the control principle diagram
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[[File:T--SZPT-CHINA--RS.png|500px|center|]]
 
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===Usage and Biology===
 
===Usage and Biology===
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K3487007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3487007 SequenceAndFeatures</partinfo>
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==2020 SZPT-CHINA==
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===Result===
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We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following.
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[[File:T--SZPT-CHINA--RS2.png|500px|center|]]
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The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally.
  
 
===References===
 
===References===
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【2】Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
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Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3487007 parameters</partinfo>
 
<partinfo>BBa_K3487007 parameters</partinfo>
 
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Revision as of 08:39, 20 October 2020


Lysine riboswitch

Description

Riboswitches are metabolite-sensitive elements found in mRNAs that control gene expression through a regulatory secondary structural switch.RS is a Lysine riboswitch that Regulated by lysine. The following is the control principle diagram

T--SZPT-CHINA--RS.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 256

2020 SZPT-CHINA

Result

We put the lysine riboswitch connected sfGFP engineered bacteria in different concentrations of lysine for 24 hours, and then tested the fluorescent expression of the bacteria. The results are shown in the following.

T--SZPT-CHINA--RS2.png

The results show that the bacteria are in the lysine at a concentration of 30μmol/mL. Fluorescence expression was significantly inhibited, indicating that the lysine riboswitch can work normally.

References

Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.