Difference between revisions of "Part:BBa K3402051"
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Revision as of 02:01, 20 October 2020


yeGFP expression cassette with NLS

This device is composed of two homologous arms upPxa1(BBa_K3402014) and doPxa1(BBa_K3402015), promoter Ptef1(BBa_K3402007), reporter gene yeGFP(BBa_K3402000), NLS(BBa_K3402008), terminator Tsyn7(BBa_K3402001), promoter Pgpd(BBa_K3402006), hygromycin resistance gene hph(BBa_K3402012) and terminator Tcbh2(BBa_K3402020).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2279
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1051
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1492