Difference between revisions of "Part:BBa K3425033:Design"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3425025 short</partinfo>
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<partinfo>BBa_K3425033 short</partinfo>
  
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<partinfo>BBa_K3425025 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3425033 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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Spacer provides optimal distance for E. coli when Type IIS iGEM Standard assembly is implemented.
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<html>
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This part is designed for the
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<a href="https://parts.igem.org/Help:Standards/Assembly/Type_IIS">Type IIS iGEM standard assembly</a>
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and the spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when this standard assembly (RFC1000) is used. You can find useful design templates and more information about how to use this standard in the
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<a href="https://2020.igem.org/Team:UofUppsala/Engineering#TypeIIS">Type IIS guidebook</a>
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of Uppsala 2020 team.
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</html>
  
 
===Source===
 
===Source===
  
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iGEM Registry of Standard Parts BBa_B0030
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iGEM Registry of Standard Biological Parts BBa_B0030
  
 
===References===
 
===References===
  
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For aligned spacing refer: Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745
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For <b>aligned spacing</b> refer:
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Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745

Latest revision as of 11:00, 19 October 2020


RBS for Type IIS iGEM Standard Assembly


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for the Type IIS iGEM standard assembly and the spacer provides aligned spacing (the distance between RBS and ATG) of 10 nucleotides when this standard assembly (RFC1000) is used. You can find useful design templates and more information about how to use this standard in the Type IIS guidebook of Uppsala 2020 team.

Source

iGEM Registry of Standard Biological Parts BBa_B0030

References

For aligned spacing refer:

Ma, J., Campbell, A., and Karlin, S. (2002) Correlations between Shine-Dalgarno Sequences and Gene Features Such as Predicted Expression Levels and Operon Structures. J. Bacteriol. 184, 5733–5745