Difference between revisions of "Part:BBa K3693004:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3693003 short</partinfo>
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<partinfo>BBa_K3693004 short</partinfo>
  
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<partinfo>BBa_K3693003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3693004 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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There are two considerations that we have during the detailed design of this sequence. On the one hand, we acknowledged the differences between humans yeasts,doing codon optimization to this sequence in order to increase protein expression. On the other hand, we put His-tag to the sequence, which indeed helps purify proteins.
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It should be capable to stimulation the expression of kappa-casein.  
  
  
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===Source===
 
===Source===
  
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It comes from the result of PCR of genomes.
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It is synthetic.
  
 
===References===
 
===References===

Revision as of 10:38, 19 October 2020


For secreting the κ-casein outside yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It should be capable to stimulation the expression of kappa-casein.


Source

It is synthetic.

References