Difference between revisions of "Part:BBa K117000:Design"

 
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<partinfo>BBa_K117000 short</partinfo>
 
<partinfo>BBa_K117000 short</partinfo>
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===Design Notes===
 
===Design Notes===
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to be updated
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Lysis gene was commercially synthesized from the company *****.
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==Extraction of Lysis gene/PCR Oligo fragments DNA sequence
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Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.
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==Source of Orgin sequence==
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to be updated.
  
 
===Source===
 
===Source===

Revision as of 19:21, 28 October 2008

Lysis gene (promotes lysis in colicin-producing bacteria strain)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lysis gene was commercially synthesized from the company *****. ==Extraction of Lysis gene/PCR Oligo fragments DNA sequence Oligo fragments with the DNA sequence of 5'-GCAGAATTCGCGGCCGCTTCTAGAG-3' & 5'-ACTAGTAGCGGCCGCTGCAGGCT-3' were used to PCR lysis gene from the blue script cloning vector.

Source of Orgin sequence

to be updated.

Source

to be updated

References