Difference between revisions of "Part:BBa K3487001"
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<partinfo>BBa_K3487001 short</partinfo>
 
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===Description===
 
 
A long-chain protein formed by polymerizing four identical short TP-I monomer peptides.
 
A long-chain protein formed by polymerizing four identical short TP-I monomer peptides.
  
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After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides.
 
After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides.
  
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===Usage and Biology===
 
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===Sequence and Features===
 
===Sequence and Features===
  

Revision as of 09:32, 19 October 2020

TP-ⅠM,A polymer of Tachyplesin Ⅰ

A long-chain protein formed by polymerizing four identical short TP-I monomer peptides.

Usage and Biology

After the tp1 polymer is expressed, it is inactive. It will be expressed as a long-chain protein by the engineered bacteria and taken to the extracellular environment by the signal peptide and then hydrolyzed into four active short peptides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



2020 SZPT-CHINA

Characterization

By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.

By externally synthesizing an expression vector with Tachyplesin I multimers, transferring the vector into BL21 for antibiotic screening to obtain a single clone, culture it in liquid medium to OD600 and add IPTG to induce 12 hours to obtain recombinant protein, and collect After ultrafiltration and concentration, purified Tachyplesin I polymer was obtained. SDS-PAGE electrophoresis identification showed that the target protein had been obtained.