Difference between revisions of "Part:BBa K3409012"

Revision as of 07:11, 19 October 2020

Microcin PDI cluster gene for McpB

Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3409012 short</partinfo>
-Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: a promoter (), an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.
+Contains the elements necessary to express the fourth gene from the Microcin PDI gene cluster (mcpD: a homolog of hlyD, coding for part of the secretion system). The sequence is organized as follows: an RBS (BBa_J1109), the CDS for McpB (BBa_K3409007). For appropriate secretion, it must be downstream of BBa_K3409012, which contains the CDS for McpD as well as a promoter (BBa_J23118) needed for transcription of McpB.
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