Difference between revisions of "Part:BBa K3629016"

(→‎Design)
(→‎Design)
Line 8: Line 8:
 
===Design===
 
===Design===
  
−
The native signal peptide from <i>T. reesei</I> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i>
+
The native signal peptide from <i>T. reesei</I> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica.</i>
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 04:40, 19 October 2020


Modified T. reesei EGI expression construct with gibson homology sequences and 6X His tag


Usage and Biology

Design

The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2385
    Illegal EcoRI site found at 2204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2204
    Illegal NheI site found at 81
    Illegal SpeI site found at 2386
    Illegal PstI site found at 2400
    Illegal NotI site found at 7
    Illegal NotI site found at 2393
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2204
    Illegal BamHI site found at 2334
    Illegal XhoI site found at 132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2386
    Illegal EcoRI site found at 2204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2204
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2386
    Illegal PstI site found at 2400
    Illegal NgoMIV site found at 1326
  • 1000
    COMPATIBLE WITH RFC[1000]