Difference between revisions of "Part:BBa K3629005:Design"
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===Design Notes===
 
===Design Notes===
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Optimized for expression and function in <i>Yarrowia lipolytica.</i>
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Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <i>P. funiculosum</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i>
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===Source===
 
===Source===

Latest revision as of 02:48, 19 October 2020


Modified Penicillium funiculosum CBHI with 6X His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 564
    Illegal BamHI site found at 1048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from P. funiculosum was also removed so it would not interfere with fused secretion tags native to Y. lipolytica

Source

Modified genomic sequence of cellobiohydrolase I from Penicillium funiculosum.

References