Difference between revisions of "Part:BBa K3629006:Design"

 
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===Design Notes===
 
===Design Notes===
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N/A
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Codon optimized for expression and function in <i>Yarrowia lipolytica.</I> The native signal peptide from <I>N. crassa</i> was also removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i>
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===Source===
 
===Source===
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===References===
 
===References===
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Guo Z-P, Duquesne S, Bozonnet S, Cioci G, Nicaud J-M, Marty A, O’Donohue MJ. Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Biotechnology for Biofuels. 2017;10(1). doi:10.1186/s13068-017-0819-8

Latest revision as of 02:48, 19 October 2020


Neurospora crassa CBHI with Myc Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
    Illegal BglII site found at 945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression and function in Yarrowia lipolytica. The native signal peptide from N. crassa was also removed so it would not interfere with fused secretion tags native to Y. lipolytica

Source

From the genomic sequence of N. crassa, but taken from the paper by Guo et al (2017).

References

Guo Z-P, Duquesne S, Bozonnet S, Cioci G, Nicaud J-M, Marty A, O’Donohue MJ. Conferring cellulose-degrading ability to Yarrowia lipolytica to facilitate a consolidated bioprocessing approach. Biotechnology for Biofuels. 2017;10(1). doi:10.1186/s13068-017-0819-8