Difference between revisions of "Part:BBa K3629008"
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===Design=== | ===Design=== | ||
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| + | The native signal peptide from <I>T. reesei</i> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica</i> | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:45, 19 October 2020
Modified Trichoderma reesei EGI with 6X His tag
Temperature and pH optimized endoglucanase I coding sequence from Trichoderma reesei with 6x His tag.
Usage and Biology
Design
The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 523
- 1000COMPATIBLE WITH RFC[1000]