Difference between revisions of "Part:BBa K3629005"
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===Design=== | ===Design=== | ||
| + | The native signal peptide from <i>P. funiculosum</i> was removed so it would not interfere with fused secretion tags native to <i>Y. lipolytica.</i> | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 02:42, 19 October 2020
Modified Penicillium funiculosum CBHI with 6X His tag
Temperature and pH optimized cellobiohydrolase I coding sequence from Penicillium funiculosum with 6x HIS tag.
Usage and Biology
Design
The native signal peptide from P. funiculosum was removed so it would not interfere with fused secretion tags native to Y. lipolytica.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 564
Illegal BamHI site found at 1048 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 46
- 1000COMPATIBLE WITH RFC[1000]