Difference between revisions of "Part:BBa K3453164"

Revision as of 20:04, 18 October 2020

Rosewood DmTrnL-UAA Trigger 2.1 transcription unit

This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger 2.1 (BBa_K3453064) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453064 which is a trigger for the Rosewood DmTrnL-UAA Toehold Switch 2.1 (BBa_K3453054).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453154 page in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3453164 short</partinfo>
-to do
+This part is a transcription unit of the Rosewood DmTrnL-UAA Trigger 2.1 ([[Part:BBa_K3453064|BBa_K3453064]]) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
+[[File: T--Evry_Paris-Saclay--toehold-switch.png|600px]]
+Figure 1. Toehold switches principle.
+This part is a transcription unit of [[Part:BBa_K3453064|BBa_K3453064]] which is a trigger for the Rosewood DmTrnL-UAA Toehold Switch 2.1 ([[Part:BBa_K3453054|BBa_K3453054]]).
+This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.
+Full results are available on the [[Part:BBa_K3453154|BBa_K3453154]] page in the registry.
+===References===
+[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
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