Difference between revisions of "Part:BBa K3453121"

Revision as of 18:00, 18 October 2020

Rosewood DmMatK Trigger 1.1 transcription unit

This part is a transcription unit of the Rosewood DmMatK Trigger 1.1 (BBa_K3453021) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453021 which is a trigger for the Rosewood DmMatK Toehold Switch 1.1 (BBa_K3453011) and also contains the switch binding site for the Rosewood DmMatK Toehold Switch 2.3 (BBa_K3453016).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453111 and BBa_K3453116 pages in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

Sequence and Features