Difference between revisions of "Part:BBa K3453121"

 
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<partinfo>BBa_K3453121 short</partinfo>
 
<partinfo>BBa_K3453121 short</partinfo>
  
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This part is a transcription unit of the Rosewood DmMatK Trigger 1.1 ([[Part:BBa_K3453021|BBa_K3453021]]) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]]) and the strong SBa_000587 synthetic terminator ([[Part:BBa_K3453000|BBa_K3453000]]).
  
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===Usage and Biology===
 
===Usage and Biology===
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A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).
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[[File: T--Evry_Paris-Saclay--toehold-switch.png|600px]]
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Figure 1. Toehold switches principle.
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This part is a transcription unit of [[Part:BBa_K3453021|BBa_K3453021]] which is a trigger for the Rosewood DmMatK Toehold Switch 1.1 ([[Part:BBa_K3453011|BBa_K3453011]]) and was designed using the web tool developed by To ''et al.'' [2]. It also contains the switch binding site for the Rosewood DmMatK Toehold Switch 2.3 ([[Part:BBa_K3453016|BBa_K3453016]]).
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This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.
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Full results are available on the [[Part:BBa_K3453111|BBa_K3453111]] and [[Part:BBa_K3453116|BBa_K3453116]] pages in the registry.
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===References===
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[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.
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[2] To AC, Chu DH, Wang AR, Li FC, Chiu AW, Gao DY, Choi CHJ, Kong SK, Chan TF, Chan KM, Yip KY. A comprehensive web tool for toehold switch design. Bioinformatics (2018) 34, 2862-2864.
  
 
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Revision as of 17:58, 18 October 2020


Rosewood DmMatK Trigger 1.1 transcription unit

This part is a transcription unit of the Rosewood DmMatK Trigger 1.1 (BBa_K3453021) for sequence-based detection of rosewood. The transcription is controlled by the T7 promoter (BBa_K2150031) and the strong SBa_000587 synthetic terminator (BBa_K3453000).

Usage and Biology

A toehold switch is an RNA–based device containing a ribosome binding site (RBS) and an ATG start codon embedded in a hairpin structure that blocks translation initiation [1]. The hairpin can be unfolded upon binding of a trigger RNA therefore exposing the RBS and the ATG start codon permitting translation of the reporter protein (Figure 1).

T--Evry Paris-Saclay--toehold-switch.png

Figure 1. Toehold switches principle.

This part is a transcription unit of BBa_K3453021 which is a trigger for the Rosewood DmMatK Toehold Switch 1.1 (BBa_K3453011) and was designed using the web tool developed by To et al. [2]. It also contains the switch binding site for the Rosewood DmMatK Toehold Switch 2.3 (BBa_K3453016).

This part proved to be functional: the transcribed rosewood RNA was able to bind to the corresponding toehold switches and a readable output was generated.

Full results are available on the BBa_K3453111 and BBa_K3453116 pages in the registry.

References

[1] Green AA, Silver PA, Collins JJ, Yin P. Toehold switches: de-novo-designed regulators of gene expression. Cell (2014) 159, 925-939.

[2] To AC, Chu DH, Wang AR, Li FC, Chiu AW, Gao DY, Choi CHJ, Kong SK, Chan TF, Chan KM, Yip KY. A comprehensive web tool for toehold switch design. Bioinformatics (2018) 34, 2862-2864.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]