Difference between revisions of "Part:BBa K3610031"

Revision as of 16:23, 18 October 2020

BAK1 without native signal peptide / YFP

This part can be used for expressing the plant pattern recognition receptor BRI1-associated receptro kinase (BAK1) from Arabidopsis thaliana in S. cerevisiae.

The sequence of the BAK1 protein does not contain the original signal peptide sequence of the receptor (BAK-). Instead it has been replaced by the secretion signal of the alpha-Factor from S. cerevisiae. To make expression of BAK1 visible and to test observe the localization in the cell, the BAK1 coding region has been fused to a yellow fluorescent protein by a 15 amino-acid long linker.

For initiation of the expression the promoter of this part is a truncated version of the ADH1 promoter from S. cerevisiae. For termination this part has terminator sequence of the enolase 2 protein from S. cerevisiae.

Usage and Biology

Characterization

Expression of BAK1-/YFP in S. cerevisiae

After successful transformation of yeast cells we checked for expression of the protein under a confocal microscope. Expression of the construct was confirmed. We failed, however, to confirm localization at the cell membrane.

Sequence and Features