Difference between revisions of "Part:BBa K3487010"

Revision as of 13:23, 18 October 2020

A kill switch regulated by lysine concentration

This part can be used directly as a suicide switch to prevent engineered bacteria from escaping into the environment, and is suitable for a variety of common engineered bacteria, such as BL21 or DH5α.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 12
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 284
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 256

Reference

   Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
   Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.

@@ Line 12: / Line 12: @@
 <partinfo>BBa_K3487010 SequenceAndFeatures</partinfo>
+===Reference===
+    Guo Y, Quiroga C, Chen Q, et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli. Nucleic Acids Res. 2014;42(10):6448-6462.
+    Garst AD, Héroux A, Rambo RP, Batey RT. Crystal structure of the lysine riboswitch regulatory mRNA element. J Biol Chem. 2008;283(33):22347-22351.
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K3487010 parameters</partinfo>
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