Difference between revisions of "Part:BBa K3431028"

 
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<partinfo>BBa_K3431028 short</partinfo>
 
<partinfo>BBa_K3431028 short</partinfo>
  
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Toehold Switch with Invertase as Expression Protein (zz146_A) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-146 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-31 (zz146_A) (BBa_K3431012) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-146. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.
  
 
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Revision as of 10:32, 18 October 2020


zz146_A_ToeholdSwitch-Regulated Invertase

Toehold Switch with Invertase as Expression Protein (zz146_A) is an RNA-based device that is to apply as a biosensor for miRNA. This biosensor is designed to detect and reflect the amount of miRNA-146 through the expression of Beta-Fructosidase (Thermotoga Maritima MSB8) (BBa_K3431000), which can convert sucrose into glucose in a time-saving process and its result can be easily represented in the readout of glucose meter. The mechanism for detection relied on the following part - Toehold Switch for miRNA-31 (zz146_A) (BBa_K3431012) - whose restriction on the expression of invertase can be liberated upon binding with miRNA-146. Furthermore, we include T7 promoter (BBa_I719005) and terminator (BBa_K731721) to insure that our device can transcribe and translate in the environment of PURExpress in vitro protein synthesis kit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 40
    Illegal NheI site found at 1427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 40
    Illegal BglII site found at 1198
    Illegal BamHI site found at 1328
    Illegal XhoI site found at 1399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 40
    Illegal AgeI site found at 999
  • 1000
    COMPATIBLE WITH RFC[1000]