Difference between revisions of "Part:BBa K079036"
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079036 K079036]:Tet O is the Wild Type operator sequence regulating Tetracycline resistance genes. The natural sequence exhibit a tigth repression given by an high Tet R bindig affinity | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079036 K079036]:Tet O is the Wild Type operator sequence regulating Tetracycline resistance genes. The natural sequence exhibit a tigth repression given by an high Tet R bindig affinity | ||
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079037 K079037]:TetO-4C is a medium affinity operator obtained through a T-C mutation in the fourth nucleotide on both side of the symmetric consensus sequence. Disrupting this interaction the repression efficiency is about '''12''' time lower than the Wt | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079037 K079037]:TetO-4C is a medium affinity operator obtained through a T-C mutation in the fourth nucleotide on both side of the symmetric consensus sequence. Disrupting this interaction the repression efficiency is about '''12''' time lower than the Wt | ||
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[https://parts.igem.org/wiki/index.php?title=Part:BBa_K079038 K079038]: TetO-wt/4C5G is a double mutant that heavily compromises the interaction of TetR with the right half of the binding sequence. The resulting repression is quite weak, more than '''40''' times less stringent than in TetO | [https://parts.igem.org/wiki/index.php?title=Part:BBa_K079038 K079038]: TetO-wt/4C5G is a double mutant that heavily compromises the interaction of TetR with the right half of the binding sequence. The resulting repression is quite weak, more than '''40''' times less stringent than in TetO | ||
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Revision as of 14:52, 28 October 2008
Tet O operator library member
These parts have been extracted from the TeT Operator Library.
K079036:Tet O is the Wild Type operator sequence regulating Tetracycline resistance genes. The natural sequence exhibit a tigth repression given by an high Tet R bindig affinity
K079037:TetO-4C is a medium affinity operator obtained through a T-C mutation in the fourth nucleotide on both side of the symmetric consensus sequence. Disrupting this interaction the repression efficiency is about 12 time lower than the Wt
K079038: TetO-wt/4C5G is a double mutant that heavily compromises the interaction of TetR with the right half of the binding sequence. The resulting repression is quite weak, more than 40 times less stringent than in TetO
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]