Difference between revisions of "Part:BBa K3629013"

 
Line 3: Line 3:
 
<partinfo>BBa_K3629013 short</partinfo>
 
<partinfo>BBa_K3629013 short</partinfo>
  
−
x
 
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<!-- -->
+
===Design===
−
<span class='h3bb'>Sequence and Features</span>
+
 
 +
===Sequence and Features===
 
<partinfo>BBa_K3629013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3629013 SequenceAndFeatures</partinfo>
  

Revision as of 07:08, 18 October 2020


Modified PfCBHI expression construct with gibson homology sequences and 6X His tag


Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2725
    Illegal EcoRI site found at 2546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal NheI site found at 75
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NotI site found at 7
    Illegal NotI site found at 2733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal BglII site found at 1322
    Illegal BamHI site found at 2631
    Illegal XhoI site found at 87
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2726
    Illegal EcoRI site found at 2546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2546
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NgoMIV site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]